Otherwise fit a standard curve of Log 10(MW) vs X. Fit a standard curve of Log 10(MW) vs Rf. Convert X to Relative Migration: Treat last band as dye front to calculate relative migration (Rf).Delete All Bands: Delete all bands marked on the profile.Delete Selected Band: Click a band to select it and then click the button to delete it.For example, if two bands are too close to distinguish, you can reduce search window size. Note: You can click the gear icon beside the button to modify the search window's width and height which are used to detect bands. Detect Bands: Detect bands and mark their positions.In this way, you can ensure different lanes start at the same position. Align: Align the top of selected lane to the top of the 1st lane.Delete Selected Lane: Click a lane to select it and then click the button to delete it.Modify Lanes: Click the button to make the lanes resizable and draggable.Add New Lane: Draw a rectangle on the image to add a new lane.Light on Dark: If the spots on the image are lighter than the background, check this option to correctly display the profile.You can customize the size of images in the report. Notes: Check Create HTML Report checkbox to output an HTML report. Click Output Results to output a report sheet.Repeat these operations to calculate molecular weight of other unknown proteins.In the bottom left panel, click Calculate Molecular Weight button to determine unknown molecular weight by the regression equation.Define another lane that contains the unknow proteins and mark bands the same way as in Step 1.Step 2: Define a lane of unknown sample and calculate molecular weight.The fitted curve is shown in the bottom right panel. Click Fit Standard Curve to fit a curve of Log 10(MW) vs Rf (or migration distance). Select function from linear, polynomial and exponential decay.In the bottom left panel, enter know molecular weight into each row of bands.In the upper right panel, detect or add bands that mark the positions of the standard proteins in the profile.In the upper left panel, draw a rectangle on the image to define a lane that contains the standard proteins.Step 1: Define a lane of standard sample and fit a standard curve.There are two steps in the determination of molecular weight.Note: Lanes in the image should be vertical and are scanned from the top to the bottom. Click the App icon to load an image and a dialog with 4 panels is opened.An icon will appear in the Apps gallery window. This App fits a standard curve to relate the known molecular weights of proteins in a set of standards (y-axis) to their relative migration (x-axis), and estimates molecular weights of unknow proteins.ĭownload the file 'GelMWAnalyzer.opx', and then drag-and-drop onto the Origin workspace.

Protein molecular weight calculator